VPS52 Polyclonal antibody, PBS Only

VPS52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11662-2-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 11662-2-AP (same clone as 11662-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

  IP result of anti-VPS52 (IP:11662-2-AP, 3ug; Detection:11662-2-AP 1:1000) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11662-2-AP (same clone as 11662-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11662-2-AP (VPS52 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

  Immunofluorescent analysis of HepG2 cells using 11662-2-AP (VPS52 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

---

Product Information

11662-2-PBS targets VPS52 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS52 fusion protein Ag2180
• Full Name: vacuolar protein sorting 52 homolog (S. cerevisiae)
• Calculated Molecular Weight: 723 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC032108
• Gene Symbol: VPS52
• Gene ID (NCBI): 6293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).