VPS45 Polyclonal antibody, PBS Only

VPS45 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12006-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12006-1-AP (same clone as 12006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12006-1-AP (same clone as 12006-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

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Product Information

12006-1-PBS targets VPS45 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS45 fusion protein Ag2658
• Full Name: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Calculated Molecular Weight: 570 aa, 65 kDa
• GenBank Accession Number: BC012932
• Gene Symbol: VPS45
• Gene ID (NCBI): 11311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)