VPS41 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS41 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13869-1-AP

Synonyms

HVPS41, hVps41p, HVSP41, S53, VPS41

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 13869-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13869-1-AP (VPS41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13869-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13869-1-AP (VPS41 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13869-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13869-1-AP (VPS41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13869-1-AP (VPS41 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13869-1-AP (VPS41 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, HepG2 cells, human brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Product Information

13869-1-AP targets VPS41 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS41 fusion protein Ag4760
• Full Name: vacuolar protein sorting 41 homolog (S. cerevisiae)
• Calculated Molecular Weight: 854 aa, 98 kDa
• Observed Molecular Weight: 94-100 kDa
• GenBank Accession Number: BC044851
• Gene Symbol: VPS41
• Gene ID (NCBI): 27072
• RRID: AB_2215349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. VPS41 (vacuolar protein sorting 41) is the human ortholog of yeast Vps41 protein. Yeast Vps41 is a subunit of AP3 (heterotetrameric adaptor protein complex 3) and HOPS (homotypic fusion and protein transport) complexes and is involved in trafficking of proteins from the late Golgi to the vacuole. In mammalian cells, Vps41 was found to be a member of HOPS complex, mediating the trafficking of proteins to lysosomes (PMID: 23173123). It has been reported that human VPS41 has a HOPS-independent function in the LAMP carrier pathway and also participates in lysosomal maturation, probably as part of the HOPS complex (PMID: 23322049).

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,CoIP	Dev Cell; ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.; Authors - Guangyan Miao ; KD Validated
human	WB	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R
human	WB	J Biol Chem; Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.; Authors - Rik van der Kant
human	WB	J Cell Sci; The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.; Authors - Magdalena Banach-Orłowska ; KD Validated