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  • KD/KO Validated

VPS39 Polyclonal antibody

VPS39 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16219-1-AP

Synonyms

hVam6p, KIAA0770, TLP, VAM6, Vam6/Vps39 like protein, VPS39



Tested Applications

Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human small intestine tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16219-1-AP targets VPS39 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS39 fusion protein Ag9267
Full Name vacuolar protein sorting 39 homolog (S. cerevisiae)
Calculated Molecular Weight 886 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC015817
Gene Symbol VPS39
Gene ID (NCBI) 23339
RRIDAB_11043179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.

Protocols

Product Specific Protocols
IHC protocol for VPS39 antibody 16219-1-APDownload protocol
IP protocol for VPS39 antibody 16219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang
IP

Cell Host Microbe

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Authors - Philipp Walch
human

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanIF

Dev Cell

ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress

Authors - Di Chen
  • KD Validated
human

Curr Biol

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Authors - Anna Ulbricht
humanIF

Cell Rep

Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes

Authors - Ariane Schleinitz