VPS37A Monoclonal antibody, PBS Only

VPS37A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9A4

Cat no : 66327-1-PBS

Synonyms

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

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Validation Data Gallery

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  WB analysis of SW 1990 using 66327-1-Ig (same clone as 66327-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66327-1-PBS targets VPS37A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VPS37A fusion protein Ag19108
• Full Name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC022363
• Gene Symbol: VPS37A
• Gene ID (NCBI): 137492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.