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  • Western Blot (WB) analysis of mouse brain tissue using VPS28 Polyclonal antibody (15478-1-AP)

    WB analysis of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS28 Polyclonal antibody (15478-1-AP)

    WB analysis using 15478-1-AP (same clone as 15478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VPS28 Polyclonal antibody (15478-1-AP)

    IP experiment of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

    IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS28 Polyclonal antibody (15478-1-AP)

    IF Staining of HepG2 using 15478-1-AP (same clone as 15478-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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Product Information

15478-1-PBS targets VPS28 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS28 fusion protein Ag7784 Predict reactive species
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC006485
Gene Symbol VPS28
Gene ID (NCBI) 51160
RRIDAB_2878144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK41
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.