VPS28 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

VPS28 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15478-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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  WB analysis using 15478-1-AP (same clone as 15478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

  IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15478-1-AP (same clone as 15478-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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Product Information

15478-1-PBS targets VPS28 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS28 fusion protein Ag7784
• Full Name: vacuolar protein sorting 28 homolog (S. cerevisiae)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC006485
• Gene Symbol: VPS28
• Gene ID (NCBI): 51160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.