VPS18 Monoclonal antibody

VPS18 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67590-1-Ig
Clone No.2E10G11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, ELISA

hVPS18, KIAA1475, PEP3, VPS18

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67590-1-Ig targets VPS18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VPS18 fusion protein Ag29954 Predict reactive species
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
RRIDAB_2882798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS18 antibody 67590-1-IgDownload protocol
IHC protocol for VPS18 antibody 67590-1-IgDownload protocol
IF protocol for VPS18 antibody 67590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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