VPS15 Polyclonal antibody, PBS Only

VPS15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17894-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 17894-1-AP (same clone as 17894-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17894-1-AP (same clone as 17894-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17894-1-AP (same clone as 17894-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

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Product Information

17894-1-PBS targets VPS15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS15 fusion protein Ag12221
• Full Name: phosphoinositide-3-kinase, regulatory subunit 4
• Calculated Molecular Weight: 1358 aa, 153 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC110318
• Gene Symbol: PIK3R4
• Gene ID (NCBI): 30849
• RRID: AB_2163630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).