VPS13C Polyclonal antibody

VPS13C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29844-1-AP

Synonyms

DKFZp686E0570, FLJ21361, KIAA1421, VPS13C

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Validation Data Gallery

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  WB analysis using 29844-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 29844-1-AP (VPS13C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

29844-1-AP targets VPS13C in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS13C fusion protein Ag31069
• Full Name: vacuolar protein sorting 13 homolog C (S. cerevisiae)
• Calculated Molecular Weight: 422 kDa
• Observed Molecular Weight: 422 kDa
• GenBank Accession Number: NM_017684
• Gene Symbol: VPS13C
• Gene ID (NCBI): 54832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS13C (vacuolar protein sorting 13 homolog C) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C, and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Publications

Species	Application	Title
human	WB,IF	bioRxiv; Lysosome damage triggers acute formation of ER to lysosomes membrane tethers mediated by the bridge-like lipid transport protein VPS13C; Authors - Xinbo Wang