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VPRBP Recombinant antibody, PBS Only (Capture)

VPRBP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241028B6

Cat no : 83901-3-PBS

Synonyms

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPRBP Recombinant antibody (83901-3-RR)

    WB analysis using 83901-3-RR (same clone as 83901-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83901-3-RR (VPRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPRBP Recombinant antibody (83901-3-RR)

    IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-3-RR, Clone: 241028B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPRBP Recombinant antibody (83901-3-RR)

    IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-3-RR, Clone: 241028B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00852-2

    Cytometric bead array standard curve of MP00852-2

    Cytometric bead array standard curve of MP00852-2, VPRBP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83901-3-PBS. Detection antibody: 83901-4-PBS. Standard: Ag2184. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83901-3-RR

    Affinity and Kinetic Characterization of 83901-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83901-3-RR against Human VPRBP were performed. The affinity constant is 0.538 nM.

Product Information

83901-3-PBS targets VPRBP as part of a matched antibody pair:

MP00852-2: 83901-3-PBS capture and 83901-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VPRBP fusion protein Ag2184
Full Name Vpr (HIV-1) binding protein
Calculated Molecular Weight 1506 aa, 169 kDa
Observed Molecular Weight169 kDa
GenBank Accession NumberBC022792
Gene Symbol VPRBP
Gene ID (NCBI) 9730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).