VISTA Recombinant antibody, PBS Only

• 

  WB analysis of human peripheral blood platelets using 83919-4-RR (same clone as 83919-4-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83919-4-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83919-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83919-4-RR against Human VISTA were performed. The affinity constant is 57.9 nM.

VISTA {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83919-4-PBS

Clone No.241003A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

---

Product Information

83919-4-PBS targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.