HeLa cells were subjected to SDS PAGE followed by western blot with 83919-3-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 83919-3-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 83919-3-RR
Biolayer interferometry (BLl) kinetic assays of 83919-3-RR against Human VISTA were performed. The affinity constant is 6.56 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.
HeLa cells were subjected to SDS PAGE followed by western blot with 83919-3-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 83919-3-RR
Biolayer interferometry (BLl) kinetic assays of 83919-3-RR against Human VISTA were performed. The affinity constant is 6.56 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
VISTA Recombinant antibody
Catalog Number
83919-3-RR
Citations
-
Dilutions
WB : 1:500-1:2000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
