VISTA Monoclonal antibody, PBS Only (Detector)

VISTA Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5C3

Cat no : 68676-2-PBS

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50058-1

  Sandwich ELISA standard curve of MP50058-1, VISTA Monoclonal Matched Antibody Pair - PBS only. 68676-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0017. 68676-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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Product Information

68676-2-PBS targets VISTA as part of a matched antibody pair:

MP50058-1: 68676-1-PBS capture and 68676-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=6.58 x 10^-11M
  K_Off=3.67 x 10^-6M
  K_On=5.58 x 10^4M
• Immunogen: Recombinant protein
• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.