VIP Polyclonal antibody

VIP Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 16233-1-AP

Synonyms

VIP peptides, Intestinal peptide PHV-42, Intestinal peptide PHM-27

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Validation Data Gallery

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  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Calretinin antibody (66496-1-Ig, Clone: 2D7A9, red).

• 

  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Calretinin antibody (66496-1-Ig, Clone: 2D7A9, red).

• 

  IF Staining of COLO 320 using 16233-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 16233-1-AP (VIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue, rat small intestine tissue, mouse brain tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue
• Positive IF/ICC detected in: COLO 320 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

16233-1-AP targets VIP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VIP fusion protein Ag8933
• Full Name: vasoactive intestinal peptide
• Calculated Molecular Weight: 169 aa, 19 kDa
• GenBank Accession Number: BC009794
• Gene Symbol: VIP
• Gene ID (NCBI): 7432
• RRID: AB_2878233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vasoactive intestinal peptide (VIP), a short peptide containing 28 amino acids belonging to the secretin-glucagon family, is initially isolated from the gastrointestinal tract as a potent vasodilator peptide. VIP was initially identified in normal nervous tissue and neurons and was subsequently recognized as a neurotransmitter widely distributed in various tissues. The wide distribution of VIP determines its involvement in a range of biological activities, such as gut motility, hormonal regulation, circadian rhythms, immune responses, and carcinogenesis. The general physiologic effects of VIP include vasodilation, anti-inflammatory actions, cell proliferation, hormonal secretion, regulation of gastric motility, and smooth muscle relaxation; therefore, VIP has emerged as a promising drug candidate for the treatment of several diseases.

Publications

Species	Application	Title
mouse	IHC	Immun Inflamm Dis; Vasoactive intestinal peptide exerts therapeutic action by regulating PTEN in a model of Sjögren's disease; Authors - Yixi Li
mouse	IF	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	IF	Cell Rep; PMID: 31509750; Authors - Jing Xue
rat	WB	Biomolecules; Postweaning Development Influences Endogenous VPAC1 Modulation of LTP Induced by Theta-Burst Stimulation: A Link to Maturation of the Hippocampal GABAergic System; Authors - Marta Gil
rat	WB	J Neurosci Res; Mismatch novelty exploration training shifts VPAC1 receptor-mediated modulation of hippocampal synaptic plasticity by endogenous VIP in male rats; Authors - Fatima Aidil-Carvalho

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

VIP (in red) worked nicely on IF in human paraffin brain sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

VIP Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:16233-1-AP)