VHL Polyclonal antibody

VHL Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (3)

Cat no : 16538-1-AP

Synonyms

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1, Von Hippe-Lindau

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Validation Data Gallery

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  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:100 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human liver cancer using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16538-1-AP (VHL antibody) at dilution of 1:100 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human kidney using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16538-1-AP (VHL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16538-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16538-1-AP (VHL antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16538-1-AP

  Immunofluorescent analysis of HepG2 cells, using VHL antibody 16538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

16538-1-AP targets VHL in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VHL fusion protein Ag9843
• Full Name: von Hippel-Lindau tumor suppressor
• Calculated Molecular Weight: 172 aa, 20 kDa
• GenBank Accession Number: BC058831
• Gene Symbol: VHL
• Gene ID (NCBI): 7428
• RRID: AB_2878273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

Publications

Species	Application	Title
human,mouse	IHC	Respir Res; Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin.; Authors - Changhui Yu
human		Oncotarget; The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.; Authors - Fabao Liu
human	IHC	Front Oncol; Risk Scores Based on Six Survival-Related RNAs in a Competing Endogenous Network Composed of Differentially Expressed RNAs Between Clear Cell Renal Cell Carcinoma Patients Carrying Wild-Type or Mutant Von Hippel-Lindau Serve Well to Predict Malignancy and Prognosis.; Authors - Rui Zhu
human,mouse	IHC	Front Mol Biosci; Network-Based Expression Analyses and Experimental Verifications Reveal the Involvement of STUB1 in Acute Kidney Injury.; Authors - Yanting Shi
human,mouse	IHC,IF	Cancer Med; UBE2S promotes malignant properties via VHL/HIF-1α and VHL/JAK2/STAT3 signaling pathways and decreases sensitivity to sorafenib in hepatocellular carcinoma; Authors - Junyi Wu
human		World J Gastroenterol; Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways; Authors - Zhan-Xue Zhao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jingwen (Verified Customer) (01-27-2020)

This antibody worked very well on the cancer samples by performing western blot and immunofluorescence.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Cancer tissue

FH

Jie (Verified Customer) (01-27-2020)

achieved positive staining in liver cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200

FH

Lunfeng (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,