Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibody

VEGFR2/CD309 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240214F12

Cat no : 98027-1-RR

Synonyms

Kdr, Krd 1, Flk1, Flk 1, 6130401C07

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Validation Data Gallery

• 

  FC experiment of bEnd.3 using 98027-1-RR

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse VEGFR2/CD309 Rabbit Recombinant Antibody (98027-1-RR, Clone: 240214F12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98027-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98027-1-RR against Mouse VEGFR-2/Kdr were performed. The affinity constant is 2.92 nM.

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Tested Applications

• Positive FC detected in: bEnd.3 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98027-1-RR is VEGFR2/CD309 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: kinase insert domain protein receptor
• Calculated Molecular Weight: 150KD
• GenBank Accession Number: NM_010612.2
• Gene Symbol: VEGFR-2
• Gene ID (NCBI): 16542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

VEGFR2, also named as CD309, KDR and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.