Anti-Human VEGFR2/CD309 Rabbit Recombinant Antibody

VEGFR2/CD309 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230238B11

Cat no : 98009-1-RR

Synonyms

KDR, VEGFR2, VEGFR2/KDR, Protein-tyrosine kinase receptor flk-1, FLK-1

---

Validation Data Gallery

• 

  FC experiment of HUVEC using 98009-1-RR

  1x10^6 HUVEC cells were surface stained with 0.25 ug Anti-Human VEGFR2/CD309 (98009-1-RR, Clone: 230238B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98009-1-RR against Human VEGFR2/CD309 were performed. The affinity constant is 30.6 nM.

---

Tested Applications

• Positive FC detected in: HUVEC cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98009-1-RR targets VEGFR2/CD309 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Calculated Molecular Weight: 152kd
• GenBank Accession Number: NM_002253
• Gene Symbol: KDR
• Gene ID (NCBI): 3791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

VEGFR2, also named as CD309, KDR and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.