CoraLite®594-conjugated VEGFA Monoclonal antibody

• Featured Product
• KD/KO Validated

VEGFA Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E2H9

Cat no : CL594-66828

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66828

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 VEGFA antibody (CL594-66828, Clone: 2E2H9 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL594-66828

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated VEGFA Monoclonal antibody (CL594-66828, Clone:2E2H9)(red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66828 targets VEGFA in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: VEGFA fusion protein Ag13500
• Full Name: vascular endothelial growth factor A
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC065522
• Gene Symbol: VEGFA
• Gene ID (NCBI): 7422
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.