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VEGFA Recombinant antibody, PBS Only (Detector)

VEGFA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242072G9

Cat no : 84687-2-PBS

Synonyms

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using VEGFA Recombinant antibody (84687-2-RR)

    WB analysis of Recombinant protein using 84687-2-RR (same clone as 84687-2-PBS)

    Recombinant protein VEGF121 (Eg1263) was subjected to SDS PAGE followed by western blot with 84687-2-RR (VEGFA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84687-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01495-1

    Cytometric bead array standard curve of MP01495-1

    Cytometric bead array standard curve of MP01495-1, VEGF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84687-1-PBS. Detection antibody: 84687-2-PBS. Standard: Eg1263. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84687-2-PBS

    Affinity and Kinetic Characterization of 84687-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84687-2-PBS against Human VEGF121 was performed. The affinity constant is 0.882 nM.

Product Information

84687-2-PBS targets VEGFA as part of a matched antibody pair:

MP01495-1: 84687-1-PBS capture and 84687-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name vascular endothelial growth factor A
GenBank Accession NumberNM_001171628.1
Gene Symbol VEGFA
Gene ID (NCBI) 7422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).