Anti-Human VE-cadherin/CD144 Rabbit Recombinant Antibody, PBS Only

VE-cadherin/CD144 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240755B2

Cat no : 98071-1-PBS

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Validation Data Gallery

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  FC experiment of HUVEC using 98071-1-RR (same clone as 98071-1-PBS)

  1x10^6 HUVEC cells were stained with 0.25 ug Anti-Human VE-cadherin/CD144 Rabbit Recombinant Antibody (98071-1-RR, Clone:240755B2) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98071-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98071-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98071-1-RR against Human VE-cadherin/CD144 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 98071-1-PBS is VE-cadherin/CD144 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cadherin 5, type 2 (vascular endothelium)
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: NM_001795.5
• Gene Symbol: VE-cadherin
• Gene ID (NCBI): 1003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).