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Mouse VE-cadherin/CD144 Recombinant antibody, PBS Only

VE-cadherin/CD144 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241953A12

Cat no : 84529-4-PBS

Synonyms

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Recombinant antibody (84529-4-RR)

    WB analysis using 84529-4-RR (same clone as 84529-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84529-4-RR (VE-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84529-4-PBS in a different storage buffer formulation.

Product Information

The immunogen of 84529-4-PBS is VE-cadherin/CD144 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name cadherin 5
Calculated Molecular Weight88kDa
Observed Molecular Weight125-130 kDa, 90-100 kDa
GenBank Accession NumberNM_009868.4
Gene Symbol Cdh5
Gene ID (NCBI) 12562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).