CoraLite®594-conjugated VE-cadherin/CD144 Monoclonal antibody

VE-cadherin/CD144 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Publications

3

CloneNo.

4F9B10

Cat no : CL594-66804

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-66804

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-66804

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:50.

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Tested Applications

• Positive IF-P detected in: human breast cancer tissue, human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 3 publications below

Product Information

CL594-66804 targets VE-cadherin/CD144 in WB, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VE-cadherin/CD144 fusion protein Ag27501
• Full Name: cadherin 5, type 2 (vascular endothelium)
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: NM_001795
• Gene Symbol: VE-cadherin
• Gene ID (NCBI): 1003
• RRID: AB_2920043
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). E-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

Publications

Species	Application	Title
human	WB,IF	Biochem Biophys Res Commun; Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage; Authors - Jun He
human	IF	Biomacromolecules; Interpenetrating Polymer Network HA/Alg-RGD Hydrogel: An Equilibrium of Macroscopic Stability and Microscopic Adaptability for 3D Cell Growth and Vascularization; Authors - Yuanshan Liu
human	IF	Biochem Biophys Res Commun; Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission; Authors - Xi Mei