Mouse VE-cadherin Recombinant antibody, PBS Only (Capture)

VE-cadherin Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241953H6

Cat no : 84529-3-PBS

Synonyms

VE Cad, Cdh5, Cd144, cadherin 5, 7B4

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01386-3

  Sandwich ELISA standard curve of MP01386-3, Mouse VE-cadherin/CD144 Recombinant Matched Antibody Pair - PBS only. 84529-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0921. 84529-6-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

• 

  Sample test of MP01386-3

  The mean VE-cadherin/CD144 concentration was determined to be 3,858.35 ng/mL in mouse heart cell extract based on a 1.20 mg/mL extract load and 709.13 ng/mL in bEnd.3 cell extract based on a 1.80 mg/mL extract load.

• 

  IF Staining of bEnd.3 using 84529-3-RR (same clone as 84529-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using VE-cadherin antibody (84529-3-RR, Clone: 241953H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84529-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01386-2

  Cytometric bead array standard curve of MP01386-2, MOUSE VE-cadherin/CD144 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84529-3-PBS. Detection antibody: 84529-2-PBS. Standard: Eg0921. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84529-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84529-3-RR against Mouse VE-cadherin were performed. The affinity constant is 1.22 nM.

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Product Information

84529-3-PBS targets VE-cadherin as part of a matched antibody pair:

MP01386-2: 84529-3-PBS capture and 84529-2-PBS detection (validated in Cytometric bead array)

MP01386-3: 84529-3-PBS capture and 84529-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 5
• Calculated Molecular Weight: 88kDa
• GenBank Accession Number: NM_009868.4
• Gene Symbol: Cdh5
• Gene ID (NCBI): 12562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).