VE-cadherin/CD144 Recombinant antibody

VE-cadherin/CD144 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240755D8

Cat no : 83766-2-RR

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Validation Data Gallery

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  WB analysis using 83766-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83766-2-RR (VE-cadherin/CD144 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83766-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-2-RR (VE-cadherin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83766-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-2-RR (VE-cadherin/CD144 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 83766-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin antibody (83766-2-RR, Clone: 240755D8 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83766-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83766-2-RR against Human VE-cadherin were performed. The affinity constant is 0.273 nM.

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Tested Applications

• Positive WB detected in: HUVEC cells, human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83766-2-RR is VE-cadherin/CD144 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cadherin 5, type 2 (vascular endothelium)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 130 kDa, 100 kDa
• GenBank Accession Number: NM_001795.5
• Gene Symbol: VE-cadherin
• Gene ID (NCBI): 1003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).