VE-cadherin/CD144 Recombinant antibody, PBS Only (Capture)

VE-cadherin/CD144 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240755A6

Cat no : 83766-1-PBS

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00752-3

  Sandwich ELISA standard curve of MP00752-3, Human VE-cadherin Recombinant Matched Antibody Pair - PBS only. 83766-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1200. 83766-4-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL

• 

  Sample test of MP00752-3

  Serum of sixteen humans was measured. The human SIRPA concentration of detected samples was determined to be 3,148.2 ng/mL with a range of 2,070.2 - 4,839.1 pg/mL.

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  Sample test of MP00752-3

  HUVEC were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin, and measured 175.74 ng/mL. JAR were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin, and measured 4.13 ng/mL.

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  Cytometric bead array standard curve of MP00752-3

  Cytometric bead array standard curve of MP00752-3, VE-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-1-PBS. Detection antibody: 83766-4-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

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Product Information

83766-1-PBS targets VE-cadherin/CD144 as part of a matched antibody pair:

MP00752-3: 83766-1-PBS capture and 83766-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cadherin 5, type 2 (vascular endothelium)
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: NM_001795.5
• Gene Symbol: VE-cadherin
• Gene ID (NCBI): 1003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.