VDAC3 Recombinant antibody

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  WB analysis using 82666-14-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82666-14-RR (VDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 82666-14-RR

  WB result of VDAC3 antibody (82666-14-RR; 1:4000; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells.

• 

  IHC staining of human ovary cancer using 82666-14-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82666-14-RR (VDAC3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

VDAC3 Recombinant Antibody for WB, IHC, ELISA

Cat No. 82666-14-RR

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

CoraLite® Plus 750

Size/Concentration: 

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Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293T cells, NIH/3T3 cells, HeLa cells, A431 cells, mouse heart tissue, mouse testis tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82666-14-RR targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 3
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_005662
• Gene Symbol: VDAC3
• Gene ID (NCBI): 7419
• RRID: AB_3670525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y277
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.