VDAC2 Recombinant antibody, PBS Only

VDAC2 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241569C3

Cat no : 84225-3-PBS

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3

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Validation Data Gallery

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  IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VDAC2 antibody (84225-3-RR, Clone: 241569C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-3-RR, Clone:241569C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 84225-3-PBS is VDAC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VDAC2 fusion protein Ag2266
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 294 aa, 32 kDa
• GenBank Accession Number: BC000165
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)