Anti-Mouse VCAM-1/CD106 Rabbit Recombinant Antibody

VCAM-1/CD106 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240597C2

Cat no : 98059-1-RR

Synonyms

CD106, VCAM-1, Vcam1, Vcam 1, 240597C2

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using 98059-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse VCAM-1/CD106 Rabbit Recombinant Antibody (98059-1-RR, Clone:240597C2) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD11b. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98059-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98059-1-RR against Mouse VCAM-1/CD106 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98059-1-RR is VCAM-1/CD106 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• GenBank Accession Number: CAA47989.1
• Gene Symbol: VCAM-1
• Gene ID (NCBI): 22329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).