VCAM-1/CD106 Recombinant antibody

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  WB analysis of 3T3-L1 using 83719-1-RR

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 83719-1-RR (VCAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 83719-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83719-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83719-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83719-1-RR against Mouse VCAM-1 were performed. The affinity constant is 0.383 nM.

VCAM-1/CD106 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83719-1-RR

Clone No.240597B2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

CD106, VCAM-1, Vcam1, Vcam 1, 240597B2

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Tested Applications

• Positive WB detected in: 3T3-L1 cells
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83719-1-RR targets VCAM-1/CD106 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: CAA47989.1
• Gene Symbol: VCAM-1
• Gene ID (NCBI): 22329
• RRID: AB_3671321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29533
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).