VCAM-1/CD106 Monoclonal antibody, PBS Only

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  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1/CD106 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1/CD106 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1/CD106 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66294-1-Ig (same clone as 66294-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66294-1-PBS in a different storage buffer formulation.

VCAM-1/CD106 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Cat No. 66294-1-PBS

Clone No.1B12F12

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Synonyms

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

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Product Information

66294-1-PBS targets VCAM-1/CD106 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24697

  Product name: Recombinant human VCAM-1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 341-739 aa of BC017276

  Sequence: ESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV

• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 739 aa, 81 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC017276
• Gene Symbol: VCAM-1
• Gene ID (NCBI): 7412
• RRID: AB_2881677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19320
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).