Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 21924-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21924-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21924-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21924-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21924-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21924-1-AP
IP result of anti-VAV2 (IP:21924-1-AP, 4ug; Detection:21924-1-AP 1:2000) with HeLa cells lysate 1520 ug.
Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 21924-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21924-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 21924-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV2 antibody (21924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV2 antibody (21924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, mouse brain tissue, A431 cells, NIH/3T3 cells, HeLa cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells
Positive IP detected in
HeLa cells
Positive IHC detected in
human kidney tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:5000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Mesenchymal stem cells promote endothelial progenitor cell migration, vascularization, and bone repair in tissue-engineered constructs via activating CXCR2-Src-PKL/Vav2-Rac1.
Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 21924-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21924-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21924-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21924-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21924-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21924-1-AP (VAV2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas using 21924-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 21924-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21924-1-AP (VAV2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 21924-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21924-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21924-1-AP (VAV2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 21924-1-AP
IP result of anti-VAV2 (IP:21924-1-AP, 4ug; Detection:21924-1-AP 1:2000) with HeLa cells lysate 1520 ug.
IF/ICC Figures
IF Staining of HeLa using 21924-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VAV2 antibody (21924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
VAV2 Polyclonal antibody
Catalog Number
21924-1-AP
Citations
3
Dilutions
WB : 1:1000-1:5000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
