VAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

VAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22016-1-AP

Synonyms

VAT1, VATI

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Validation Data Gallery

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  WB analysis using 22016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 22016-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22016-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22016-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 22016-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 22016-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 22016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 22016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: C6 cells, Jurkat cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 22016-1-AP is VAT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAT1 fusion protein Ag16652
• Full Name: vesicle amine transport protein 1 homolog (T. californica)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC015041
• Gene Symbol: VAT1
• Gene ID (NCBI): 10493
• RRID: AB_11182282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	Cancer Immunol Immunother; Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.; Authors - Pei Yang ; KD Validated
human	WB	Oncol Rep; High expression of VAT1 is a prognostic biomarker and predicts malignancy in glioblastoma.; Authors - Xia Shan