VASP Polyclonal antibody

• Featured Product
• KD/KO Validated

VASP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 13472-1-AP

Synonyms

Vasodilator-stimulated phosphoprotein

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Validation Data Gallery

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  WB analysis using 13472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13472-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13472-1-AP (VASP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13472-1-AP

  IP result of anti-VASP (IP:13472-1-AP, 4ug; Detection:13472-1-AP 1:10000) with HEK-293 cells lysate 920 ug.

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  IHC staining of human colon using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13472-1-AP (VASP antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13472-1-AP (VASP antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin cancer using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 13472-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13472-1-AP (VASP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 13472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VASP antibody (13472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 13472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using VASP antibody (13472-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 13472-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug VASP Polyclonal antibody (13472-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, mouse spleen tissue, HEK-293 cells, Hela cells, C6 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human normal colon, human lung tissue, human skin cancer tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A549 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

13472-1-AP targets VASP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VASP fusion protein Ag4266
• Full Name: vasodilator-stimulated phosphoprotein
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: 46 kDa, 50 kDa
• GenBank Accession Number: BC038224
• Gene Symbol: VASP
• Gene ID (NCBI): 7408
• RRID: AB_2213418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.

Publications

Species	Application	Title
human	IF	Nat Commun; Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.; Authors - Thibault Courtheoux
human	WB	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB, IF, IHC	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li ; KD Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang
human	WB	Oncol Rep; TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway.; Authors - Wen Liu ; KD Validated
human	WB,IHC	Acta Histochem; Exploring the oncogenic role of RGS19 in bladder cancer progression and prognosis; Authors - Lei Yan