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CoraLite® Plus 488-conjugated VAPA Monoclonal antibody

VAPA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F10F9

Cat no : CL488-67845

Synonyms

VAMP associated protein A, VAMP A, hVAP 33, 33 kDa VAMP-associated protein, 33 kDa VAMP associated protein

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated VAPA Monoclonal anti (CL488-67845)

    FC experiment of HepG2 using CL488-67845

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VAPA (CL488-67845, Clone:1F10F9) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67845 targets VAPA in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAPA fusion protein Ag7819
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 VAPA antibody CL488-67845Download protocol
Standard Protocols
Click here to view our Standard Protocols