VAMP7/TI-VAMP Polyclonal antibody, PBS Only

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  WB analysis using 22268-1-AP (same clone as 22268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22268-1-AP (same clone as 22268-1-PBS)

  IP result of anti-VAMP7/TI-VAMP (IP:22268-1-AP, 4ug; Detection:22268-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 22268-1-AP (same clone as 22268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAMP7/TI-VAMP antibody (22268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22268-1-AP (same clone as 22268-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 22268-1-AP (VAMP7/TI-VAMP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22268-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VAMP7/TI-VAMP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 22268-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

VAMP7, TI VAMP, Tetanus-insensitive VAMP, Tetanus insensitive VAMP, Synaptobrevin-like protein 1

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Product Information

22268-1-PBS targets VAMP7/TI-VAMP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: vesicle-associated membrane protein 7
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: NM_005638
• Gene Symbol: VAMP7
• Gene ID (NCBI): 6845
• RRID: AB_11182718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51809
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

VAMP7, also named SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during the transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is necessary for calcium-regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.