ChromoTek Ubiquitin-Trap Magnetic Agarose

The ChromoTek Ubiquitin-Trap Magnetic Agarose consists of an anti-Ubiquitin Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of ubiquitin and ubiquitinylated proteins from mammalian, plant and yeast cell extracts.

Specificity

monomeric ubiquitin, ubiquitin chains, and ubiquitinylated proteins

Reactivity

Pan-reactive. Tested with human, mouse, hamster, dog, spinach, yeast ubiquitin

Applications

IP, CoIP

Type

Nanobody

Conjugate

Magnetic Agarose

Publications

2

Cat no : utma

Synonyms

polyubiquitin B, UBB, ubiquitin, ubiquitin B

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Validation Data Gallery

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  The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from human (HEK293T), mouse (C2C12), and hamster (CHO) cell lines treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.

• 

  The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from dog (MDCK) cells, spinach (Spinacia oleracea), and baker's yeast (Saccharomyces cerevisiae) treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.

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Product Information

The ChromoTek Ubiquitin-Trap Magnetic Agarose consists of an anti-Ubiquitin Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of ubiquitin and ubiquitinylated proteins from mammalian, plant and yeast cell extracts.

• Description: Immunoprecipitation of ubiquitin and ubiquitinylated proteins with anti-Ubiquitin Nanobody conjugated to magnetic agarose beads; ;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, CoIP
• Specificity/Target: Binds to monomeric ubiquitin, ubiquitin chains, and ubiquitinylated proteins.; Ubiquitin chains may be linked via lysines; linkages via lysines such as K11, K48 and K63 are compatible with binding by the Ubiquitin-Trap (further linkage types also likely to be compatible, but not tested).
• Tested Reactivity: Human, Mouse, Hamster, Dog, Spinach, Yeast
• Conjugate: Magnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli)
• Wash buffer compatibility: 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Camelid
• Affinity: The K_D for monomeric ubiquitin is 90 nM. The apparent affinity is even higher for ubiquitin chains due to avidity effects.
• Compatibility with mass spectrometry: The Ubiquitin-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2943578
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Other Formats

Ubiquitin-Trap Magnetic Agarose Kit

Publications

Application	Title
	bioRxiv; A human papillomavirus 16 E2-TopBP1 dependent SIRT1-p300 acetylation switch regulates mitotic viral and human protein levels; Authors - Apurva T Prabhakar
IP	Nat Commun; OTUD6 deubiquitination of RPS7/eS7 on the free 40 S ribosome regulates global protein translation and stress; Authors - Sammy Villa