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Ubiquilin 1 Recombinant antibody, PBS Only (Capture)

Ubiquilin 1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242092E9

Cat no : 84649-2-PBS

Synonyms

UBQLN1, hPLIC-1, hPLIC1, hPLIC 1, DSK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ubiquilin 1 Recombinant antibody (84649-2-RR)

    WB analysis using 84649-2-RR (same clone as 84649-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84649-2-RR (Ubiquilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84649-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ubiquilin 1 Recombinant antibody (84649-2-RR)

    IF Staining of HeLa using 84649-2-RR (same clone as 84649-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ubiquilin 1 antibody (84649-2-RR, Clone: 242092E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84649-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01462-1

    Cytometric bead array standard curve of MP01462-1

    Cytometric bead array standard curve of MP01462-1, Ubiquilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84649-2-PBS. Detection antibody: 84649-1-PBS. Standard:Ag17530. Range: 0.469-30 ng/mL.

  • Cytometric bead array standard curve of MP01462-2

    Cytometric bead array standard curve of MP01462-2

    Cytometric bead array standard curve of MP01462-2, Ubiquilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84649-2-PBS. Detection antibody: 84649-3-PBS. Standard: Ag17530. Range: 0.469-30 ng/mL.

  • Affinity and Kinetic Characterization of 84649-2-RR

    Affinity and Kinetic Characterization of 84649-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84649-2-RR against Human Ubiquilin 1 were performed. The affinity constant is 1.08 nM.

Product Information

84649-2-PBS targets Ubiquilin 1 as part of a matched antibody pair:

MP01462-1: 84649-2-PBS capture and 84649-1-PBS detection (validated in Cytometric bead array)

MP01462-2: 84649-2-PBS capture and 84649-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ubiquilin 1 fusion protein Ag17530
Full Name ubiquilin 1
Calculated Molecular Weight 589 aa, 63 kDa
Observed Molecular Weight 63-71 kDa
GenBank Accession NumberBC010066
Gene Symbol Ubiquilin 1
Gene ID (NCBI) 29979
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.