Ubiquilin 1 Polyclonal antibody, PBS Only

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  WB analysis using 22126-1-AP (same clone as 22126-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22126-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 22126-1-AP (same clone as 22126-1-PBS)

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22126-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22126-1-AP (same clone as 22126-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ubiquilin 1 antibody (22126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22126-1-PBS in a different storage buffer formulation.

Ubiquilin 1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 22126-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

UBQLN1, PLIC 1, hPLIC-1, hPLIC1, hPLIC 1

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Product Information

22126-1-PBS targets Ubiquilin 1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ubiquilin 1 fusion protein Ag17530
• Full Name: ubiquilin 1
• Calculated Molecular Weight: 589 aa, 63 kDa
• Observed Molecular Weight: 63-71 kDa
• GenBank Accession Number: BC010066
• Gene Symbol: Ubiquilin 1
• Gene ID (NCBI): 29979
• RRID: AB_2879000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UMX0
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.