USP9X Recombinant antibody, PBS Only

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  WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 81892-1-RR (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

  WB result of USP9X antibody (81892-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP9X transfected HEK-293 cells. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

  IP result of anti-USP9X (IP:81892-1-RR, 4ug; Detection:81892-1-RR 1:3000) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 81892-1-RR (same clone as 81892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81892-1-RR (same clone as 81892-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP9X Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 81892-1-PBS

Clone No.1B23

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

FAM, FAF, EC:3.4.19.12, DFFRX, Deubiquitinating enzyme FAF-X

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Product Information

81892-1-PBS targets USP9X in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin specific peptidase 9, X-linked
• Calculated Molecular Weight: 292 kDa
• Observed Molecular Weight: 260-290 kDa
• GenBank Accession Number: NM_001039590
• Gene Symbol: USP9X
• Gene ID (NCBI): 8239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93008
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.