USP8 Recombinant antibody

USP8 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240016D3

Cat no : 83172-2-RR

Synonyms

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis using 83172-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83172-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of U2OS using 83172-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83172-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83172-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HCT 116 cells, K-562 cells, A549 cells, HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83172-2-RR targets USP8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: USP8 fusion protein Ag27143
• Full Name: ubiquitin specific peptidase 8
• Calculated Molecular Weight: 1118 aa, 128 kDa
• GenBank Accession Number: BC110590
• Gene Symbol: USP8
• Gene ID (NCBI): 9101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.