USP8 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 27791-1-AP

Synonyms

Ubiquitin thioesterase 8, Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy

---

Validation Data Gallery

• 

  WB analysis using 27791-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27791-1-AP (USP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27791-1-AP

  WB result of USP8 antibody (27791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP8 transfected HeLa cells.

• 

  WB analysis of MCF-7 using 27791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27791-1-AP (USP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 27791-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27791-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

27791-1-AP targets USP8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP8 fusion protein Ag27143
• Full Name: ubiquitin specific peptidase 8
• Calculated Molecular Weight: 1118 aa, 128 kDa
• Observed Molecular Weight: 128 kDa
• GenBank Accession Number: BC110590
• Gene Symbol: USP8
• Gene ID (NCBI): 9101
• RRID: AB_2880973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Differ; Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer; Authors - Hanshen Yang
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
mouse,rat	WB	Aging Dis; Exogenous H2S Promoted USP8 Sulfhydration to Regulate Mitophagy in the Hearts of db/db Mice.; Authors - Yu Sun
human	WB	Arch Biochem Biophys; Mitochondria-targeted Catalase induced cell malignant transformation by the downregulation of p53 protein stability via USP28/miR-200b/PP2A-Cα axis; Authors - Chaowei Wen
human	WB,IHC	Cancer Manag Res; Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion.; Authors - Xu Jing ; KD Validated
human	WB	Front Oncol; Knockdown of USP8 inhibits prostate cancer cell growth, proliferation, and metastasis and promotes docetaxel’s activity by suppressing the NF-kB signaling pathway; Authors - Md. Tariqul Islam ; KD Validated