USP42 Polyclonal antibody

USP42 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18811-1-AP

Synonyms

USP42

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Validation Data Gallery

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  WB analysis of mouse brain using 18811-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18811-1-AP (USP42 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18811-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18811-1-AP (USP42 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18811-1-AP (USP42 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18811-1-AP (USP42 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18811-1-AP targets USP42 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin specific peptidase 42
• Calculated Molecular Weight: 145 kDa
• Observed Molecular Weight: 146 kDa
• GenBank Accession Number: BC136656
• Gene Symbol: USP42
• Gene ID (NCBI): 84132
• RRID: AB_10603481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP42(ubiquitin carboxyl-terminal hydrolase 42) is also named as deubiquitinating enzyme 42 and belongs to the peptidase C19 family. It consists of 1,324 amino acids with a predicted molecular weight of 146 kDa and contains the conserved Cys, Asp (I), His and Asn/Asp (II) domains defined as one of characteristics for deubiquitinating enzymes, which may play an important role in mouse embryogenesis and spermatogenesis(PMID:16904385). This protein has 2 isoforms produced by alternative splicing. This antibody is specific to USP42.

Publications

Species	Application	Title
human	WB	Cell Death Differ; USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.; Authors - Shuyan Liu