USP40 Polyclonal antibody

USP40 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27217-1-AP

Synonyms

USP 40, Ubiquitin-specific-processing protease 40, Ubiquitin thioesterase 40, Ubiquitin carboxyl-terminal hydrolase 40, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis using 27217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27217-1-AP (USP40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27217-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27217-1-AP (USP40 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27217-1-AP targets USP40 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP40 fusion protein Ag25976
• Full Name: ubiquitin specific peptidase 40
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC013305
• Gene Symbol: USP40
• Gene ID (NCBI): 55230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP40 (ubiquitin specific peptidase 40), also known as ubiquitin thioesterase 40, deubiquitinating enzyme 40 or ubiquitin carboxyl-terminal hydrolase 40, is a 1235 amino acid protein that belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. USP40 transcript is ubiquitously expressed but abundant in the kidney, heart, spleen, liver, and testis. Within the kidney, USP40 is expressed in glomerular endothelial cells during development (PMID:28148530).