USP38 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP38 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17767-1-AP

Synonyms

Deubiquitinating enzyme 38, HP43.8KD, KIAA1891, Ubiquitin thiolesterase 38, USP38

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Validation Data Gallery

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  WB analysis of HEK-293T using 17767-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17767-1-AP (USP38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 17767-1-AP

  IP result of anti-USP38 (IP:17767-1-AP, 4ug; Detection:17767-1-AP 1:500) with K-562 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

17767-1-AP targets USP38 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP38 fusion protein Ag12172
• Full Name: ubiquitin specific peptidase 38
• Calculated Molecular Weight: 1042 aa, 117 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC068975
• Gene Symbol: USP38
• Gene ID (NCBI): 84640
• RRID: AB_2213383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin specific peptidase 38 (USP38) is a negative regulator of type I IFN signaling. USP38 can strongly induced the degradation of the active form of TBK1 after viral infection. USP38 may play a critical role in the maintenance of the balance between innate immune responses and tolerance through dynamic ubiquitin editing of TBK1 in response to viral infection and perhaps other infectious diseases or cancer. (PMID:27692986)

Publications

Species	Application	Title
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen ; KO Validated
human	IHC,WB,CoIP	Oncogenesis; USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.; Authors - Wei Zhan ; KD Validated
human	WB,IHC,IP	Am J Cancer Res; Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase.; Authors - Zhi Zheng ; KD Validated
human	WB	Int J Biochem Cell Biol; The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc.; Authors - Zhijun Xu
mouse,rat	WB,IP,IF	Mol Med; USP38 exacerbates pressure overload-induced left ventricular electrical remodeling; Authors - Yucheng Pan
mouse	WB	Int Immunopharmacol; Methylprednisolone alleviates lung injury in sepsis by regulating miR-151-5p/USP38 pathway; Authors - Zhize Yuan