USP25 Polyclonal antibody

USP25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28817-1-AP

Synonyms

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

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Validation Data Gallery

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  WB analysis of HEK-293T using 28817-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 28817-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 28817-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28817-1-AP (USP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 28817-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 28817-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28817-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28817-1-AP (USP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, PC-3 cells, RAW 264.7 cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28817-1-AP targets USP25 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP25 fusion protein Ag30463
• Full Name: ubiquitin specific peptidase 25
• Calculated Molecular Weight: 125 kDa
• Observed Molecular Weight: 122-138 kDa
• GenBank Accession Number: BC015930
• Gene Symbol: USP25
• Gene ID (NCBI): 29761
• RRID: AB_2918207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as a deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.