USP25 Polyclonal antibody, PBS Only

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  WB analysis using 12199-1-AP (same clone as 12199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 12199-1-AP (same clone as 12199-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

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  IP experiment of RAW 264.7 using 12199-1-AP (same clone as 12199-1-PBS)

  IP result of anti-USP25 (IP:12199-1-AP, 4ug; Detection:12199-1-AP 1:300) with RAW 264.7 cells lysate 1600ug. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP25 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

12199-1-PBS targets USP25 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP25 fusion protein Ag2837
• Full Name: ubiquitin specific peptidase 25
• Calculated Molecular Weight: 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC015930
• Gene Symbol: USP25
• Gene ID (NCBI): 29761
• RRID: AB_2212771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHP3
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.