USP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10392-1-AP

Synonyms

USP9, UBP41, Ubiquitin thiolesterase 2, ubiquitin specific peptidase 2, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis using 10392-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10392-1-AP

  NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10392-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10392-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10392-1-AP (USP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10392-1-AP (USP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10392-1-AP (USP2 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of mouse embryo using 10392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 10392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse brain tissue, mouse kidney tissue, MCF-7 cells, HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse embryo tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

10392-1-AP targets USP2 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP2 fusion protein Ag0591
• Full Name: ubiquitin specific peptidase 2
• Calculated Molecular Weight: 41 kDa, 69 kDa
• Observed Molecular Weight: 68 kDa, 41-45 kDa
• GenBank Accession Number: BC002955
• Gene Symbol: USP2
• Gene ID (NCBI): 9099
• RRID: AB_2212432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing. This antibody is specific to the isoform 1(USP2a) of USP2.

Publications

Species	Application	Title
human	WB,IHC	Gastroenterology; Dual inhibition of CDK4/6 and XPO1 induces senescence with acquired vulnerability to CRBN-based PROTAC drugs; Authors - Hui Wang
human	WB	FASEB J; Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation.; Authors - Kitamura Hiroshi H
rat	WB	J Cell Mol Med; Ubiquitin-specific protease 2 regulates Ang Ⅱ-induced cardiac fibroblasts activation by up-regulating cyclin D1 and stabilizing β-catenin in vitro.; Authors - Qiong Xu ; KD Validated
mouse	WB	Mol Nutr Food Res; 3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity.; Authors - Hee Yang
mouse	WB,IHC	Mediators Inflamm; Overexpression of Ubiquitin-Specific Protease 2 (USP2) in the Heart Suppressed Pressure Overload-Induced Cardiac Remodeling.; Authors - Junhui Xing
human,mouse	WB,IF,CoIP	J Cancer Res Clin Oncol; USP2 promotes cell proliferation and metastasis in choroidal melanoma via stabilizing Snail; Authors - Chao Wei