Tested Applications
| Positive WB detected in | MCF-7 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21362-1-AP targets USP17 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP17 fusion protein Ag15824 Predict reactive species |
| Full Name | ubiquitin specific peptidase 17 |
| Calculated Molecular Weight | 530 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC100991 |
| Gene Symbol | USP17 |
| Gene ID (NCBI) | 391627 |
| RRID | AB_3669365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP17, also known as DUB3, is a deubiquitinating enzyme (DUB) that plays a key role in regulating the balance between ubiquitination and deubiquitination of proteins. This balance is essential for protein degradation, transport, localization, and activity. USP17 is inducibly expressed in response to cytokine, chemokine, and epidermal growth factor (EGF) stimulation, and several studies have shown that it is required for cell proliferation and migration. Aberrantly expressed USP17 has been associated with inflammation, cell motility, development of T helper 17 (Th17) cells and carcinogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP17 antibody 21362-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
