USP16 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP16 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14055-1-AP

Synonyms

Ubiquitin-specific-processing protease 16, Ubiquitin-processing protease UBP-M, Ubiquitin thioesterase 16, Ubiquitin carboxyl-terminal hydrolase 16, MSTP039

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Validation Data Gallery

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  WB analysis using 14055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14055-1-AP (USP16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14055-1-AP

  IP result of anti-USP16 (IP:14055-1-AP, 5ug; Detection:14055-1-AP 1:1000) with HeLa cells lysate 880ug.

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  IHC staining of human liver using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human brain tissue, human spleen tissue, human ovary tissue, human kidney tissue, human heart tissue, human testis tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14055-1-AP targets USP16 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP16 fusion protein Ag4981
• Full Name: ubiquitin specific peptidase 16
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC030777
• Gene Symbol: USP16
• Gene ID (NCBI): 10600
• RRID: AB_2272832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP16 (Ubiquitin specific peptidase 16) was originally identified as a DUB of histone H2A which is implicated in cell cycle progression and HOXD10 gene expression (PMID:17914355). The knockout study has reported that depletion of USP16 results in early embryonic lethality in mice (PMID:24784029). Moreover, USP16 mediated the deubiquitination and stabilization of Drp1 through its direct interaction with Drp1 (PMID:37488647).

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase; Authors - Nadine Sen Nkwe
mouse	WB,CoIP	Arthritis Res Ther; Deubiquitinase USP16 induces gouty arthritis via Drp1-dependent mitochondrial fission and NLRP3 inflammasome activation; Authors - Qingdong Wang ; KD Validated
human	WB,IF	J Cell Sci; A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase.; Authors - Nadine Sen Nkwe ; KD Validated
human	IHC	Rheumatology (Oxford); Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.; Authors - Christine Galant
human	WB,IP	Nat Commun; FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice; Authors - Gaozan Tong ; KD Validated