USP14 Monoclonal antibody, PBS Only

USP14 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B12G3

Cat no : 67746-1-PBS

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

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Validation Data Gallery

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  WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67746-1-Ig (same clone as 67746-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP14 antibody (67746-1-Ig, Clone: 3B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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Product Information

67746-1-PBS targets USP14 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP14 fusion protein Ag6312
• Full Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC003556
• Gene Symbol: USP14
• Gene ID (NCBI): 9097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.